Endothelial Cell Growth Medium 2 lacks Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains Insulin-like Growth Factor (Long R3 IGF) and Vascular Endothelial Growth Factor (VEGF). Generally, VEGF leads to higher endothelial cell proliferation in culture. But, because of its multiple effects on cell metabolism, it may also interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium, which contains no VEGF.
Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.
To view a list of references where this medium was used with other species/cell types please click here.
Components:
Media supplement | Final supplement concentration |
---|---|
Fetal Calf Serum | 0.02 ml / ml |
Epidermal Growth Factor (recombinant human) | 5 ng / ml |
Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml |
Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml |
Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml |
Ascorbic Acid | 1 µg / ml |
Heparin | 22.5 μg / ml |
Hydrocortisone | 0.2 µg / ml |
Note: For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2. Heparin, included in all our other Endothelial Cell Growth Media formulations, may reduce transfection efficiency.
Promokine代理
,简介:蚂蚁淘生物公司做为Promokine代理,专业经营原装进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗等。
Promokine代理的产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞治疗、临床应用等领域。做为代理,我们郑重承诺:保证原装进口,如出现运输质量问题,我们可以包退换货。
代理,所能提供产品如下:
Recombinant Human ULBP-2 Fc Chimera, CF (50 UG),R&D 货号:1298-UL-050,规格:50 UG
Recombinant Human NKG2D Fc Chimera, CF (50 UG),R&D 货号:1299-NK-050,规格:50 UG
Recombinant Mouse MD-1 (50 UG),R&D 货号:130-MD-050,规格:50 UG
Recombinant Mouse MD-1, CF (50 UG),R&D 货号:130-MD-050/CF,规格:50 UG
Recombinant Human MICA Fc Chimera, CF (50 UG),R&D 货号:1300-MA-050,规格:50 UG
Recombinant Mouse Galectin-7 (50 UG),R&D 货号:1304-GA-050,规格:50 UG
Recombinant Mouse Galectin-7, CF (50 UG),R&D 货号:1304-GA-050/CF,规格:50 UG
Recombinant Human Galectin-8 (50 UG),R&D 货号:1305-GA-050,规格:50 UG
Recombinant Human Galectin-8, CF (50 UG),R&D 货号:1305-GA-050/CF,规格:50 UG
Recombinant Rat Erythropoietin (10 UG),R&D 货号:1306-RE-010,规格:10 UG
Recombinant Rat Erythropoietin, CF (10 UG),R&D 货号:1306-RE-010/CF,规格:10 UG
Recombinant Mouse Frizzled-2 Fc Chimera, CF (50 UG),R&D 货号:1307-FZ-050,规格:50 UG
Recombinant Mouse Notch-3 Fc Chimera, CF (50 UG),R&D 货号:1308-NT-050,规格:50 UG
Recombinant Mouse Prolactin R Fc Chimera, CF (50 UG),R&D 货号:1309-PR-050,规格:50 UG
Recombinant Human CCL23/MPIF-1 (aa 46-120) (25 UG),R&D 货号:131-M1-025,规格:25 UG
Recombinant Human CCL23/MPIF-1 (aa 46-120), CF (25 UG),R&D 货号:131-M1-025/CF,规格:25 UG
Recombinant Human u-Plasminogen Activator/Urokinase, CF (10 UG),R&D 货号:1310-SE-010,规格:10 UG
Recombinant Rat IFN-alpha (100000 UN),R&D 货号:13100-1,规格:100000 UN
Recombinant Human Sonic Hedgehog/Shh, N-Terminus (25 UG),R&D 货号:1314-SH-025,规格:25 UG
Recombinant Human Sonic Hedgehog/Shh, N-Terminus, CF (25 UG),R&D 货号:1314-SH-025/CF,规格:25 UG
Recombinant Mouse Nodal (25 UG),R&D 货号:1315-ND-025,规格:25 UG
Recombinant Mouse Nodal, CF (25 UG),R&D 货号:1315-ND-025/CF,规格:25 UG
Recombinant Rat IL-22 (10 UG),R&D 货号:1316-RL-010,规格:10 UG
Recombinant Rat IL-22, CF (10 UG),R&D 货号:1316-RL-010/CF,规格:10 UG
Recombinant Mouse Thrombopoietin R Fc Chimera, CF (50 UG),R&D 货号:1317-TR-050,规格:50 UG
Recombinant Rhesus Macaque IL-1 beta/IL-1F2 (10 UG),R&D 货号:1318-RL-010,规格:10 UG
Recombinant Rhesus Macaque IL-1 beta/IL-1F2, CF (10 UG),R&D 货号:1318-RL-010/CF,规格:10 UG
Recombinant Human TL1A/TNFSF15 (10 UG),R&D 货号:1319-TL-010,规格:10 UG
Recombinant Human TL1A/TNFSF15, CF (10 UG),R&D 货号:1319-TL-010/CF,规格:10 UG
Bovine FGF acidic (25 UG),R&D 货号:132-FA-025,规格:25 UG