The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.
Components:The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium
Note: The serum- and xeno-free formulation means non-availability of attachment factors. Hence, coating with an appropriate adhesion factor is a pre-requisite. For the establishment of the culture conditions, it is recommended to test fibronectin (e.g. Human Fibronectin Solution (C-43060)) and vitronectin (e.g. Vitronectin (C-69201)) coating.
Key features:
- Suitable for long-term routine culture of adherently growing established human cancer cell lines
- Compatible with most commonly used human cancer cell lines
- Xeno-free and serum-free formulation
List of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:
| Tissue | Tested Cell Lines | Cell Lines Origin | Remarks |
|---|---|---|---|
| Brain | BV2 | immortalized murine primary microglial cells | Coat with Fibronectin:1 µg/cm2 |
| Breast | MCF-7 | pleural effusion of metastatic human breast adenocarcinoma | Coat with Fibronectin:1 µg/cm2 |
| Colon | HT-29 | human colon adenocarcinoma | Coat with Vitronectin:0.5 µg/cm2 |
| Connective tissue | HT1080 | human fibrosarcoma | Coat with Fibronectin:1 µg/cm2 |
| Liver | HepG2 | hepatocellular carcinoma of the human liver | Coat with Vitronectin:0.5 µg/cm2 |
| Lung | A-549 | human lung carcinoma | Coat with Vitronectin:0.5 µg/cm2 |
| Prostate | LNCaP | lymph node metastasis of human prostate adenocarcinoma | 3D culture in C-28070 is recommended |
| Peripheral blood | MV-4-11 | Human acute myelogenous leukemia (suspension) | No coating required |
| Bone marrow | KG-1 | Human acute myelogenous leukemia (suspension) | No coating required |
| Kidney | ACHN | Human Renal Cell Carcinoma | Coat with Fibronectin:1 µg/cm2 |
| Brain | A172 | Human Glioblastoma | Coat with Fibronectin:1 µg/cm2 |
| Skeletal muscle | C2C12 | Mouse Myoblasts | Coat with Fibronectin:1 µg/cm2 |
| Skin | B16-F10 | Mouse Melanoma | Coat with Fibronectin:1 µg/cm2 |
| Abelson murine leukemia virus-induced tumor | RAW264.7 | Mouse Macrophage | Coat with Fibronectin:1 µg/cm2 |
| Brain, neuroectodermal | Neuro-2a | Mouse Neuroblastoma | Coat with Fibronectin:1 µg/cm2 |
Promokine代理
，简介：蚂蚁淘生物公司做为Promokine代理，专业经营原装进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗等。
Promokine代理的产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞治疗、临床应用等领域。做为代理,我们郑重承诺：保证原装进口，如出现运输质量问题，我们可以包退换货。
代理，所能提供产品如下：
Recombinant Human ULBP-2 Fc Chimera, CF (50 UG),R&D 货号：1298-UL-050,规格:50 UG
Recombinant Human NKG2D Fc Chimera, CF (50 UG),R&D 货号：1299-NK-050,规格:50 UG
Recombinant Mouse MD-1 (50 UG),R&D 货号：130-MD-050,规格:50 UG
Recombinant Mouse MD-1, CF (50 UG),R&D 货号：130-MD-050/CF,规格:50 UG
Recombinant Human MICA Fc Chimera, CF (50 UG),R&D 货号：1300-MA-050,规格:50 UG
Recombinant Mouse Galectin-7 (50 UG),R&D 货号：1304-GA-050,规格:50 UG
Recombinant Mouse Galectin-7, CF (50 UG),R&D 货号：1304-GA-050/CF,规格:50 UG
Recombinant Human Galectin-8 (50 UG),R&D 货号：1305-GA-050,规格:50 UG
Recombinant Human Galectin-8, CF (50 UG),R&D 货号：1305-GA-050/CF,规格:50 UG
Recombinant Rat Erythropoietin (10 UG),R&D 货号：1306-RE-010,规格:10 UG
Recombinant Rat Erythropoietin, CF (10 UG),R&D 货号：1306-RE-010/CF,规格:10 UG
Recombinant Mouse Frizzled-2 Fc Chimera, CF (50 UG),R&D 货号：1307-FZ-050,规格:50 UG
Recombinant Mouse Notch-3 Fc Chimera, CF (50 UG),R&D 货号：1308-NT-050,规格:50 UG
Recombinant Mouse Prolactin R Fc Chimera, CF (50 UG),R&D 货号：1309-PR-050,规格:50 UG
Recombinant Human CCL23/MPIF-1 (aa 46-120) (25 UG),R&D 货号：131-M1-025,规格:25 UG
Recombinant Human CCL23/MPIF-1 (aa 46-120), CF (25 UG),R&D 货号：131-M1-025/CF,规格:25 UG
Recombinant Human u-Plasminogen Activator/Urokinase, CF (10 UG),R&D 货号：1310-SE-010,规格:10 UG
Recombinant Rat IFN-alpha (100000 UN),R&D 货号：13100-1,规格:100000 UN
Recombinant Human Sonic Hedgehog/Shh, N-Terminus (25 UG),R&D 货号：1314-SH-025,规格:25 UG
Recombinant Human Sonic Hedgehog/Shh, N-Terminus, CF (25 UG),R&D 货号：1314-SH-025/CF,规格:25 UG
Recombinant Mouse Nodal (25 UG),R&D 货号：1315-ND-025,规格:25 UG
Recombinant Mouse Nodal, CF (25 UG),R&D 货号：1315-ND-025/CF,规格:25 UG
Recombinant Rat IL-22 (10 UG),R&D 货号：1316-RL-010,规格:10 UG
Recombinant Rat IL-22, CF (10 UG),R&D 货号：1316-RL-010/CF,规格:10 UG
Recombinant Mouse Thrombopoietin R Fc Chimera, CF (50 UG),R&D 货号：1317-TR-050,规格:50 UG
Recombinant Rhesus Macaque IL-1 beta/IL-1F2 (10 UG),R&D 货号：1318-RL-010,规格:10 UG
Recombinant Rhesus Macaque IL-1 beta/IL-1F2, CF (10 UG),R&D 货号：1318-RL-010/CF,规格:10 UG
Recombinant Human TL1A/TNFSF15 (10 UG),R&D 货号：1319-TL-010,规格:10 UG
Recombinant Human TL1A/TNFSF15, CF (10 UG),R&D 货号：1319-TL-010/CF,规格:10 UG
Bovine FGF acidic (25 UG),R&D 货号：132-FA-025,规格:25 UG
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