Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin or adult skin from different locations including the face, the breasts, the abdomen, and the thighs.

Melanocytes are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes.The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation. It is produced and stored in melanosomes, which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.

PromoCell Normal Human Epidermal Melanocytes 2 are isolated using the serum-free and PMA-free Melanocyte Growth Medium M2. Since PMA is a tumor promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M2 and also using this medium for cultivation.

Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.

NEW: Our NHEM 2 are now also available from HLA-typed donors.

Available formats:

• Cryopreserved: Cryogenic vial containing 500.000 viable cells.
• Proliferating: >500.000 viable cells shipped in growth medium (T25 flask).
• Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.

Promokine代理
，简介：蚂蚁淘生物公司做为Promokine代理，专业经营原装进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗等。
Promokine代理的产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞治疗、临床应用等领域。做为代理,我们郑重承诺：保证原装进口，如出现运输质量问题，我们可以包退换货。
代理，所能提供产品如下：
Recombinant Human ULBP-2 Fc Chimera, CF (50 UG),R&D 货号：1298-UL-050,规格:50 UG
Recombinant Human NKG2D Fc Chimera, CF (50 UG),R&D 货号：1299-NK-050,规格:50 UG
Recombinant Mouse MD-1 (50 UG),R&D 货号：130-MD-050,规格:50 UG
Recombinant Mouse MD-1, CF (50 UG),R&D 货号：130-MD-050/CF,规格:50 UG
Recombinant Human MICA Fc Chimera, CF (50 UG),R&D 货号：1300-MA-050,规格:50 UG
Recombinant Mouse Galectin-7 (50 UG),R&D 货号：1304-GA-050,规格:50 UG
Recombinant Mouse Galectin-7, CF (50 UG),R&D 货号：1304-GA-050/CF,规格:50 UG
Recombinant Human Galectin-8 (50 UG),R&D 货号：1305-GA-050,规格:50 UG
Recombinant Human Galectin-8, CF (50 UG),R&D 货号：1305-GA-050/CF,规格:50 UG
Recombinant Rat Erythropoietin (10 UG),R&D 货号：1306-RE-010,规格:10 UG
Recombinant Rat Erythropoietin, CF (10 UG),R&D 货号：1306-RE-010/CF,规格:10 UG
Recombinant Mouse Frizzled-2 Fc Chimera, CF (50 UG),R&D 货号：1307-FZ-050,规格:50 UG
Recombinant Mouse Notch-3 Fc Chimera, CF (50 UG),R&D 货号：1308-NT-050,规格:50 UG
Recombinant Mouse Prolactin R Fc Chimera, CF (50 UG),R&D 货号：1309-PR-050,规格:50 UG
Recombinant Human CCL23/MPIF-1 (aa 46-120) (25 UG),R&D 货号：131-M1-025,规格:25 UG
Recombinant Human CCL23/MPIF-1 (aa 46-120), CF (25 UG),R&D 货号：131-M1-025/CF,规格:25 UG
Recombinant Human u-Plasminogen Activator/Urokinase, CF (10 UG),R&D 货号：1310-SE-010,规格:10 UG
Recombinant Rat IFN-alpha (100000 UN),R&D 货号：13100-1,规格:100000 UN
Recombinant Human Sonic Hedgehog/Shh, N-Terminus (25 UG),R&D 货号：1314-SH-025,规格:25 UG
Recombinant Human Sonic Hedgehog/Shh, N-Terminus, CF (25 UG),R&D 货号：1314-SH-025/CF,规格:25 UG
Recombinant Mouse Nodal (25 UG),R&D 货号：1315-ND-025,规格:25 UG
Recombinant Mouse Nodal, CF (25 UG),R&D 货号：1315-ND-025/CF,规格:25 UG
Recombinant Rat IL-22 (10 UG),R&D 货号：1316-RL-010,规格:10 UG
Recombinant Rat IL-22, CF (10 UG),R&D 货号：1316-RL-010/CF,规格:10 UG
Recombinant Mouse Thrombopoietin R Fc Chimera, CF (50 UG),R&D 货号：1317-TR-050,规格:50 UG
Recombinant Rhesus Macaque IL-1 beta/IL-1F2 (10 UG),R&D 货号：1318-RL-010,规格:10 UG
Recombinant Rhesus Macaque IL-1 beta/IL-1F2, CF (10 UG),R&D 货号：1318-RL-010/CF,规格:10 UG
Recombinant Human TL1A/TNFSF15 (10 UG),R&D 货号：1319-TL-010,规格:10 UG
Recombinant Human TL1A/TNFSF15, CF (10 UG),R&D 货号：1319-TL-010/CF,规格:10 UG
Bovine FGF acidic (25 UG),R&D 货号：132-FA-025,规格:25 UG