PCR Mycoplasma Test Kit I/C utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of mycoplasma and acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 10-15 fg of mycoplasma DNA corresponding to 10-15 mycoplasma genomes per sample volume. The primer set is specific for the highly conserved rRNA operon, or more specifically, the 16S-rRNA coding region in the mycoplasma genome. This allows for detection of many Mollicutes strains such as M. orale, M. hyorhinis, M. arginini, M. fermentans, M. salivarium, and M. hominis, which are usually encountered as contaminants in cell cultures, but also Acholeplasma laidlawii and many other species (see complete list below). The kit does not detect the clinically relevant M. pneumoniae and Ureaplasma urealyticum which however are not known as cell culture contaminants. Eukaryotic and bacterial DNA is not amplified by using the kit. Only one protocol is needed for the detection of all mycoplasma species. The detection procedure can be performed within 3 hours. PCR Mycoplasma Test Kit I/C also provides internal control DNA (as negative control or for verifying a successful PCR run) as well as a positive control. When running the PCR with the internal control DNA, a successfully performed reaction is indicated by a 479 bp band on the agarose gel. The positive control yields a band at 270 bp.
All components required for the PCR (primer, nucleotides, control DNA, and Hot-Start Taq polymerase) come lyophilized in PCR tubes included in the kit (lyophilized Master Mix). For setting up the PCR, simply cut off the number of tubes required, rehydrate lyophilized Master Mix with provided Rehydration Buffer and add the sample.Samples can be easily prepared by boiling and centrifuging the cell culture supernatant.
Promokine代理
,简介:蚂蚁淘生物公司做为Promokine代理,专业经营原装进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗等。
Promokine代理的产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞治疗、临床应用等领域。做为代理,我们郑重承诺:保证原装进口,如出现运输质量问题,我们可以包退换货。
代理,所能提供产品如下:
Recombinant Human ULBP-2 Fc Chimera, CF (50 UG),R&D 货号:1298-UL-050,规格:50 UG
Recombinant Human NKG2D Fc Chimera, CF (50 UG),R&D 货号:1299-NK-050,规格:50 UG
Recombinant Mouse MD-1 (50 UG),R&D 货号:130-MD-050,规格:50 UG
Recombinant Mouse MD-1, CF (50 UG),R&D 货号:130-MD-050/CF,规格:50 UG
Recombinant Human MICA Fc Chimera, CF (50 UG),R&D 货号:1300-MA-050,规格:50 UG
Recombinant Mouse Galectin-7 (50 UG),R&D 货号:1304-GA-050,规格:50 UG
Recombinant Mouse Galectin-7, CF (50 UG),R&D 货号:1304-GA-050/CF,规格:50 UG
Recombinant Human Galectin-8 (50 UG),R&D 货号:1305-GA-050,规格:50 UG
Recombinant Human Galectin-8, CF (50 UG),R&D 货号:1305-GA-050/CF,规格:50 UG
Recombinant Rat Erythropoietin (10 UG),R&D 货号:1306-RE-010,规格:10 UG
Recombinant Rat Erythropoietin, CF (10 UG),R&D 货号:1306-RE-010/CF,规格:10 UG
Recombinant Mouse Frizzled-2 Fc Chimera, CF (50 UG),R&D 货号:1307-FZ-050,规格:50 UG
Recombinant Mouse Notch-3 Fc Chimera, CF (50 UG),R&D 货号:1308-NT-050,规格:50 UG
Recombinant Mouse Prolactin R Fc Chimera, CF (50 UG),R&D 货号:1309-PR-050,规格:50 UG
Recombinant Human CCL23/MPIF-1 (aa 46-120) (25 UG),R&D 货号:131-M1-025,规格:25 UG
Recombinant Human CCL23/MPIF-1 (aa 46-120), CF (25 UG),R&D 货号:131-M1-025/CF,规格:25 UG
Recombinant Human u-Plasminogen Activator/Urokinase, CF (10 UG),R&D 货号:1310-SE-010,规格:10 UG
Recombinant Rat IFN-alpha (100000 UN),R&D 货号:13100-1,规格:100000 UN
Recombinant Human Sonic Hedgehog/Shh, N-Terminus (25 UG),R&D 货号:1314-SH-025,规格:25 UG
Recombinant Human Sonic Hedgehog/Shh, N-Terminus, CF (25 UG),R&D 货号:1314-SH-025/CF,规格:25 UG
Recombinant Mouse Nodal (25 UG),R&D 货号:1315-ND-025,规格:25 UG
Recombinant Mouse Nodal, CF (25 UG),R&D 货号:1315-ND-025/CF,规格:25 UG
Recombinant Rat IL-22 (10 UG),R&D 货号:1316-RL-010,规格:10 UG
Recombinant Rat IL-22, CF (10 UG),R&D 货号:1316-RL-010/CF,规格:10 UG
Recombinant Mouse Thrombopoietin R Fc Chimera, CF (50 UG),R&D 货号:1317-TR-050,规格:50 UG
Recombinant Rhesus Macaque IL-1 beta/IL-1F2 (10 UG),R&D 货号:1318-RL-010,规格:10 UG
Recombinant Rhesus Macaque IL-1 beta/IL-1F2, CF (10 UG),R&D 货号:1318-RL-010/CF,规格:10 UG
Recombinant Human TL1A/TNFSF15 (10 UG),R&D 货号:1319-TL-010,规格:10 UG
Recombinant Human TL1A/TNFSF15, CF (10 UG),R&D 货号:1319-TL-010/CF,规格:10 UG
Bovine FGF acidic (25 UG),R&D 货号:132-FA-025,规格:25 UG