The PCR Mycoplasma Test Kit I/RT utilizes quantitative, real-time PCR (qPCR). It shows excellent sensitivity (detects approximately 10 mycoplasmas per sample volume) and specificity and allows to obtain reliable PCR results extremely fast (in “real time”). Thus, the kit is suited particularly well for routine testing/screening for mycoplasma contamination using cell culture supernatants. It contains mycoplasma-specific probes and comes with Inhibition Control DNA as well as Positive Control DNA. Defined DNA standards for quantitative Real-Time PCR are available separately.

The kit detects a wide range of mycoplasma species and is compatible with any Real-Time PCR instrument able to detect the fluorescence dyes FAM™ and HEX™. The complete detection procedure can be performed within 3 hours.

Background

Mycoplasmas are specifically detected by amplifying a highly conserved 23S rRNA coding region in the mycoplasma genome. This mycoplasma-specific amplification is detected at 520 nm (FAM™ channel).The kit includes a lyophilized, complete Master Mix containing primer and FAM™ labeled probes which allow the specific detection of all Mollicutes species so far described as contaminants of cell cultures and media components. Eukaryotic DNA is not amplified by this primer/probe system.False negative results due to PCR inhibitors or improper DNA extraction are detected by the Internal Amplification Control which can be added to the PCR master mix. The amplification of the internal amplification control is detected at 560 nm (HEX™ channel).

Kit Components PCR Mycoplasma Test Kit I/RT; Variant C– Primer&Probes Mix: containing mycoplasma specific primer set; nucleotides dATP, dCTP, dGTP and dUTP; Taq DNA Polymerase; (lyophilized master mix; for 25 or 50 reactions)– Inhibition Control Spike: containing artificial DNA fragments/plasmid DNA (lyophilized; non-infectious; for at least 25 or 50 reactions)– Positive Control Spike, containing artificial DNA-fragments/plasmid DNA (lyophilized; non-infectious; for at least 25 or 50 reactions)– Rehydration/Reaction Buffer– DNA-free Water– Instruction manual

Mollicutes species detectable with PCR Mycoplasma Test Kit I/RT, Variant C| Based on positive testing | Based on sequence alignments | Based on sequence alignments ||—————————|——————————|——————————|| Mycoplasma arginini | Mycoplasma agalactiae | Mycoplasma hyopharyngis || Mycoplasma arthritidis | Mycoplasma alligatoris | Mycoplasma hyopneumoniae || Mycoplasma fermentans | Mycoplasma alvi | Mycoplasma hyosynoviae || Mycoplasma gallisepticum | Mycoplasma amphoriforme | Mycoplasma iguanae || Mycoplasma genitalium | Mycoplasma anatis | Mycoplasma imitans || Mycoplasma hominis | Mycoplasma bovis | Mycoplasma mobile || Mycoplasma hyorhinis | Mycoplasma canis | Mycoplasma ovipneumoniae || Mycoplasma orale | Mycoplasma columbinum | Mycoplasma pulmonis || Mycoplasma penetrans | Mycoplasma conjunctivae | Mycoplasma testudineum || Mycoplasma pneumoniae | Mycoplasma crocodyli | Mycoplasma testudinis || Mycoplasma salivarium | Mycoplasma flocculare | || Mycoplasma synoviae | | || Acholeplasma laidlawii | | |

Promokine代理
，简介：蚂蚁淘生物公司做为Promokine代理，专业经营原装进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗等。
Promokine代理的产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞治疗、临床应用等领域。做为代理,我们郑重承诺：保证原装进口，如出现运输质量问题，我们可以包退换货。
代理，所能提供产品如下：
Recombinant Human ULBP-2 Fc Chimera, CF (50 UG),R&D 货号：1298-UL-050,规格:50 UG
Recombinant Human NKG2D Fc Chimera, CF (50 UG),R&D 货号：1299-NK-050,规格:50 UG
Recombinant Mouse MD-1 (50 UG),R&D 货号：130-MD-050,规格:50 UG
Recombinant Mouse MD-1, CF (50 UG),R&D 货号：130-MD-050/CF,规格:50 UG
Recombinant Human MICA Fc Chimera, CF (50 UG),R&D 货号：1300-MA-050,规格:50 UG
Recombinant Mouse Galectin-7 (50 UG),R&D 货号：1304-GA-050,规格:50 UG
Recombinant Mouse Galectin-7, CF (50 UG),R&D 货号：1304-GA-050/CF,规格:50 UG
Recombinant Human Galectin-8 (50 UG),R&D 货号：1305-GA-050,规格:50 UG
Recombinant Human Galectin-8, CF (50 UG),R&D 货号：1305-GA-050/CF,规格:50 UG
Recombinant Rat Erythropoietin (10 UG),R&D 货号：1306-RE-010,规格:10 UG
Recombinant Rat Erythropoietin, CF (10 UG),R&D 货号：1306-RE-010/CF,规格:10 UG
Recombinant Mouse Frizzled-2 Fc Chimera, CF (50 UG),R&D 货号：1307-FZ-050,规格:50 UG
Recombinant Mouse Notch-3 Fc Chimera, CF (50 UG),R&D 货号：1308-NT-050,规格:50 UG
Recombinant Mouse Prolactin R Fc Chimera, CF (50 UG),R&D 货号：1309-PR-050,规格:50 UG
Recombinant Human CCL23/MPIF-1 (aa 46-120) (25 UG),R&D 货号：131-M1-025,规格:25 UG
Recombinant Human CCL23/MPIF-1 (aa 46-120), CF (25 UG),R&D 货号：131-M1-025/CF,规格:25 UG
Recombinant Human u-Plasminogen Activator/Urokinase, CF (10 UG),R&D 货号：1310-SE-010,规格:10 UG
Recombinant Rat IFN-alpha (100000 UN),R&D 货号：13100-1,规格:100000 UN
Recombinant Human Sonic Hedgehog/Shh, N-Terminus (25 UG),R&D 货号：1314-SH-025,规格:25 UG
Recombinant Human Sonic Hedgehog/Shh, N-Terminus, CF (25 UG),R&D 货号：1314-SH-025/CF,规格:25 UG
Recombinant Mouse Nodal (25 UG),R&D 货号：1315-ND-025,规格:25 UG
Recombinant Mouse Nodal, CF (25 UG),R&D 货号：1315-ND-025/CF,规格:25 UG
Recombinant Rat IL-22 (10 UG),R&D 货号：1316-RL-010,规格:10 UG
Recombinant Rat IL-22, CF (10 UG),R&D 货号：1316-RL-010/CF,规格:10 UG
Recombinant Mouse Thrombopoietin R Fc Chimera, CF (50 UG),R&D 货号：1317-TR-050,规格:50 UG
Recombinant Rhesus Macaque IL-1 beta/IL-1F2 (10 UG),R&D 货号：1318-RL-010,规格:10 UG
Recombinant Rhesus Macaque IL-1 beta/IL-1F2, CF (10 UG),R&D 货号：1318-RL-010/CF,规格:10 UG
Recombinant Human TL1A/TNFSF15 (10 UG),R&D 货号：1319-TL-010,规格:10 UG
Recombinant Human TL1A/TNFSF15, CF (10 UG),R&D 货号：1319-TL-010/CF,规格:10 UG
Bovine FGF acidic (25 UG),R&D 货号：132-FA-025,规格:25 UG